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FITC Alpha-Synuclein Preformed Fibrils
Product Background: Alpha-Synuclein (α-Synuclein) is a presynaptic protein found to be a major component of Parkinson’s Disease (PD) aggregates and is implicated in the pathogenesis of PD and related neurodegenerative disorders.
About the product: While some fluorescent Alpha-Synuclein on the market uses intrinsic fluorescence or adds in tryptophan residues by mutations, rPeptide’s human Alpha-Synuclein has been covalently labeled with Fluorescein Isothiocyanate and assayed for fluorescence. Lysine residues of the native Alpha-Synuclein sequence were used as labeling sites, leaving the cysteine residues unaffected. This FITC labeled form of human Alpha-Synuclein was then formed into fibrils according to rPeptide’s proprietary methods. Fibril formation is determined by TEM and thioflavin assay.
Applications: The FITC Alpha-Synuclein fibrils are sold in 200mM Tris, 250mM NaCl, pH 7.4 and may be suitable for experimental use or as controls in aggregation kinetics, fibril morphology, seeding experiments, or in vivo imaging.
Categories: Preformed Fibrils, Synuclein Preformed Fibrils
Specifications
Amino Acid Sequence
Description & Background
Product Background: Alpha-Synuclein (α-Synuclein) is a presynaptic protein found to be a major component of Parkinson’s Disease (PD) aggregates and is implicated in the pathogenesis of PD and related neurodegenerative disorders.
About the product: While some fluorescent Alpha-Synuclein on the market uses intrinsic fluorescence or adds in tryptophan residues by mutations, rPeptide’s human Alpha-Synuclein has been covalently labeled with Fluorescein Isothiocyanate and assayed for fluorescence. Lysine residues of the native Alpha-Synuclein sequence were used as labeling sites, leaving the cysteine residues unaffected. This FITC labeled form of human Alpha-Synuclein was then formed into fibrils according to rPeptide’s proprietary methods. Fibril formation is determined by TEM and thioflavin assay.
Applications: The FITC Alpha-Synuclein fibrils are sold in 200mM Tris, 250mM NaCl, pH 7.4 and may be suitable for experimental use or as controls in aggregation kinetics, fibril morphology, seeding experiments, or in vivo imaging.